Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPITSENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKNEIEKDMSLIFDLVDYVSY-PQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK 4Y2O Chain:A ((3-210)) ------------------------------------NFMIYPIS-----------KDLK--NGN--SELVRVYSKSKEIQYIKIYTKKIINPGTTEEYEVDIP---------NWDGGLVVTPQKVILPAGASKSIRLTQFKIPK--KEEVYR-VYFEAVKPDSK-------TIELSVNIIYAALIRSLPSE--QNISLN---ISR---NA--KKNIIIYNNGNVRAG-VKDIYFCKSSNIDDN-CVKKAYNKNIYPEKSFDTLVNN-----NFSYVFIKLNHE-GIE-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Y2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 897 4245 4.73 21.44 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 4.73 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_4Y2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y2O-query.scw PDB file : Tito_Scwrl_4Y2O.pdb: