Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVTGIGLPFLGVIAIGVSKSS-GVYELAKR-INQTY-AMVFTILLYLVIGPFFALPRLATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLLLAFLNPLGSVN-QAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD 3GI9 Chain:C ((3-303)) --LKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAIGDNIITGALSILLWMS--YVISIALFAKGFAGYFLPL-I-APINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLLILGLFIFAGLIT--IHPSYVIPDLAPSAVSGMIFASAIFFLSY---MGFGVITNASEHIEN-----PK---KNVPRAIFISILIVMFVYVGVAISAIGNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLA---------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1440 -204579 -142.07 -691.15 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.58 3D Compatibility (PKB) : -142.07 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_3GI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GI9-query.scw PDB file : Tito_Scwrl_3GI9.pdb: