Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREKHYFNQDRNFWINV---QQNDVLAVKNLKEEYGISDEMLTYSLDKNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTT-------PNTEYVIRLIRSLL-ERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLLQLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYR---KLSEKEK----EELDDALIEARQAVE-MTNLASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQ-LPFTHSVFGWGIALIISLVLSIWMLIALWRRIR 4I0U Chain:A ((61-348)) -------------WINITGIHRTDV--VQRVGEFFGIHPLVLEDILNVHQRPKVEF--FENYVFIVLKMFTYDKNLHELESEQVSLILTKNCVLMFQEKIGDVFDPVRER-IRYNRGIIRKKRADYLLYSLID----ALVDDYFVLLEKIDDEIDVLEEEVLERPEKETVQRTHQLKRNLVEL----RKTIWPLREVLS-SLYRDVPPLIEKETVPYFRDVYDHTIQIADTVETFRDIVSGLLD----VYLSSVSNKTNEVMKVLTIIATIFMPLTFIAGIYGMNFEYMPELRWKWGYPVVLAVMGVIAVIMVVYFKKK--

General information: TITO was launched using: RESULT: Template: 4I0U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 979 13917 14.22 51.93 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 14.22 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_4I0U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I0U-query.scw PDB file : Tito_Scwrl_4I0U.pdb: