Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNS 5C42 Chain:A ((509-534)) --------------QAQPDKSESELVNQIIEQLIKKEKVY---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5C42.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 2101 80.81 80.81 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 80.81 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.711

(partial model without unconserved sides chains): PDB file : Tito_5C42.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C42-query.scw PDB file : Tito_Scwrl_5C42.pdb: