Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKEEIHMFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKTGLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPMLLPIYEGEGATTFNMFSVKVAMDLYDKLAN------VTGTKY-ENYTLTPEEVLEREPFLKKEGLKGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTDEVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFDTGKQDGRMVFAIPRENK-TYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANITLNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVEDVLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKEEYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNARRIFELA---KEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNH-LLFERDQLDEIKQPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK 2R46 Chain:A ((31-491)) ----------------------------------------------------MLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLP----HRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPE---------IKRGFEYSDCWVD----DARLVLANAQMVVRKGGEVLTRTRATSARRENGLWI-VEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVH---TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHF-KKQLSRDDIVWTYSGVRPLC-----------DDESDSPQA---------------ITRDYTLDI--HDENG---KAP---------------LLSVFGGKLTTYRKLAEHAL---EKLTPYYQGIGPAW--TKESVLPGGAIEGDR-DDYAARLRRR--YPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYE-AELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVE------------------------------------

General information: TITO was launched using: RESULT: Template: 2R46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2377 101650 42.76 226.39 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 42.76 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_2R46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R46-query.scw PDB file : Tito_Scwrl_2R46.pdb: