Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRETVEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPKDPN---DDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKEVIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEE-VEIDIEDKDIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARVYDKIQQEAQSEYDA--NRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGKLDEIVDALIMYDQTSKLEAMQNG 1GQE Chain:A ((24-361)) -YLDYDAKKERLEEVNAELEQPDVWNEPERAQALGKERSSLEAVVDTLDQMK---QGLEDVSGLLELAVEADDEETFNEAVAEL----DALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYEVEMQKKNAEKQAMEDNKSDIGWG---SQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEASL--------------

General information: TITO was launched using: RESULT: Template: 1GQE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1421 60403 42.51 181.94 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 42.51 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_1GQE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GQE-query.scw PDB file : Tito_Scwrl_1GQE.pdb: