Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKILLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDL-GAEKREKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSG-GLQGGSTLTQQLIKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETASEMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQTEYDQAVNVPVTDGL--------QEL-TQSDDNTKIVDNYVK-EVINEVQEKTDK--NVYTDGLEIYTNLDLDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLAVNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITD-APYNYEGTSTPVGNWDNQYMGTITLRQALYLSRNVPAVKLFNEV----GSDKVASFLKNLGIEY-STIHQSNAISSNTEEQDGTKYGASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDILKDTITE-GTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSISVWTGYNK-KMTPVTSESSHVASDVYRELMQYVSANVTNTDWE---MPSGLIRVGGELYYKDQYTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSEPASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR 2OLU Chain:A ((10-634)) ----------------------------------------------------------------------------KLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDY-------------GFGSEGASTLTQQVVKDAFLS-------IGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSF--YKNKDQQVGATILDSKTGGLVAISGGRDFKDVV---NRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGST--FRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASE-------FSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSV----------------------SGSIPSINVSGSQDNNTTNRSTH------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2OLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3232 218244 67.53 374.35 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 67.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_2OLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLU-query.scw PDB file : Tito_Scwrl_2OLU.pdb: