Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNVAPLYAEKI--MFQLRQANFEPVLSIV----PAGETSKSLEQAAELYDFLADND--FTRSDGILALGGGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPAGVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNE--------LKDEKDLLEHAV--------PIITATLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHAEKIGLSPTGSTAALERILQKFHLPITYDLSH-----------PDELFHAITHDKKARGSQLNIILLEKIGQAKIVPVPTETFKDYLGGL 1NR5 Chain:A ((41-372)) -------------------------------------LVTDTNIGSIYTPSFEEAFRKRAAEITPSPRLLIYNRPPGEVSKSRQTKADIEDWMLSQNPPCGRDTVVIALGGGVIGDLTGFVASTYMRGVRYVQVPTTLLAMVDSSIGGKTAIDTPLGKNLIGAIWQPTKIYIDLEFLETLPVREFINGMAEVIKTAAISSEEEFTALEENAETILKAVRREVTPGEHRFEGTEEILKARILASARHKAYVVSADEREGGLRNLLNWGHSIGHAIEAILTPQIL-HGECVAIGMVKEAELARHLGILKGVAVSRIVKCLAAYGLPTSLKDARIRKLTAGKHCSVDQLMFNMALDKKNDGPKKKIVLLSAIG-------------------

General information: TITO was launched using: RESULT: Template: 1NR5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1418 9365 6.60 31.53 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 6.60 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_1NR5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NR5-query.scw PDB file : Tito_Scwrl_1NR5.pdb: