Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNKEAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDLMTPERMEDYHRILDKNLTQALEA--QHGQFELFDLSKMVFFTSICEWAGINLSAISKDEVEKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAFANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFS----RPDLYQQLKNDFSTLQDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDSFMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEFSVPEQDWTIPFNQFPTYPNSRALLYKN 3VNO Chain:A ((13-396)) ---------------------YRFISRQCQRLGANAFESRFLLKKTNCLKGAKAAEIFYDTTRFEREGAMPVAIQKTLLGQGGVQGLDGETHRHRKQMFMGLMTPERVRALAQLFEAEWRRAVPGWTRKGEIVFYDELHEPLTRAVCAWAGVPL---PDDEAGNRAGELRALFDAAGSASPRHLWSRLARRRVDAWAKRIIEGIRAGSIGSGSGTAAYAIAWHRDRHDDLLSPHVAAVELVNVLEPTVAIAVYITFVAHALQTCSGIRAALVQQ--PDYAEL---FVQEVRRFYPFFPAVVARASQDFEWEGMAFPEGRQVVLDLYGSNHDAATWADPQEFRPERF--RAWD---EDSFNFIPQGGGDHYLGHRCPGEWIVLAIMKVAAHLLVNAMRYDVPDQDLSIDFARLPALPKS-------

General information: TITO was launched using: RESULT: Template: 3VNO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1872 -24726 -13.21 -65.41 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -13.21 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3VNO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VNO-query.scw PDB file : Tito_Scwrl_3VNO.pdb: