Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIVIGQSGSGKSTLARKIKEITDFP-----LLPLDLLWHTTDYS---IQAKKWFLQEQQLFMVSNPSWIVEGN-----YTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDM----PESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN 2EW1 Chain:A ((28-167)) -KIVLIGNAGVGKTCLVRRFTQGL-FPPGQGATIGVDFMIKTVEINGEKVKLQIWDTAGQERFRSITQSYYRSANALILTYDITCEESFRCLPE--WLREIEQYASNKVITVLVGNKIDLAERREVSQQRAEEFSEAQDMYYLE----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 533 7035 13.20 57.19 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 13.20 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_2EW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EW1-query.scw PDB file : Tito_Scwrl_2EW1.pdb: