Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCTAITYATKDH--YFGRNFDYEMSYNEVVTITPRNYRFD-FRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADY-KELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLP---LSPLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTRV 2HF0 Chain:A ((1-191)) -CTGVRFSDDEGNTYFGRNLDWSFSYGETILVTPRGYHYDTVFGAGGKAKPNAVIGVGVVMADRPMYFDCANEHGLAIAGLNFPGYASFVHEPVEGTENVATFEFPLWVARNFDSVDEVEETLRNVTLV----SQIVPGQQESLLHWFIGDGKRSIVVEQMADGMHVHHDDVDVLTNQPTFDFHMENLRNYMCVSN----

General information: TITO was launched using: RESULT: Template: 2HF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 953 -15658 -16.43 -85.10 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -16.43 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_2HF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HF0-query.scw PDB file : Tito_Scwrl_2HF0.pdb: