Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNYLDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVI--PGMGQVKDPFEQAVRNVQNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSVITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQDLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLTLFGYWCYRKFKRQKQRKKNNR 4G1H Chain:A ((16-195)) -----------------------------------------------------------IDTNTVERRIALANAYNETLSRNPLLIDPFTSK----LREYARML-----EVHEQ--IGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLPTARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTCTPYMINSHRLLVKGERIP-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4G1H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 945 -24292 -25.71 -139.61 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -25.71 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4G1H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1H-query.scw PDB file : Tito_Scwrl_4G1H.pdb: