Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKLNVTLLRFM-----EDEDFRVLTALEMAMRNH---------------------------------------------DVAPTALVERIAQLPHGGCRKRLNNLLKHKMIHHENTMYD------------GYAMKYGAY-D-YLALRTLSK--RGTCTGVGHRIGCGK----------------ESDI--------------------------ILVR---------------DEAGHECVLKLQ---RLGR---------CSFRSVARNRDYKGNGRAR----HGESWFYLSR-LASQKEYSFMKLLYDEGFPVPK----------PIDQNRHALLMELVPGTLLNNITELGDASKVYRRALELMVKLAESGLIHGDFNEFNLMITDDQRVIMIDFPQMISINHPNAS-----------ELFDRDVQNLANFFHRRFKVDTTWFPSLEKDVVRKGELDKQVYASGHFTRKQQE-DLERLMLE-----GFQRSE-----KSDDEAGSQVRDSDASDDDGDE--NASQRSDIGNA-DV-D--------------AGVAGKFTSIPGGARGGESDEEREDSSDDDA-----SEGRLLDEAMGAARERREGEIYARNT-DRNANFLPDGKINEDHVKHQIRKNIRRKDNHDFNRHLHRNVQKGRQKQKIQRQLKSSN-GGGFFD-------------------------------------- 4M0M Chain:A ((13-740)) EQKVKTVTLSFLGTGQHREKVHHILTSFHNTISEVNKDNPTVAMRMFDGPGSEPKSGDSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQKLTGNLAGEGIEHLLFEAVLYLNDIIEKNGGKLPETVNLHGFSRGADTCMRMANLLYQLYPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVEHFESTLMLHEYRPGFDPQHSGRYVIADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLNDDMNRFCRETGSLPSVGISPPIIARVGDKKEEVRTHSELSPEKRFELLCGMKENEWGYAKLTKKYHERSILSKREDYVQDSRLFVNQEHRELFKQLYPKSFNWFFEKNHGGQTKKEEVIVELKSLSEDPRYEHFFSSLAKHFQINENNIAGTLPEPSGIDRDEKSSFGQPPVRDRLSYLQHSLTSIANYYHYHCDEKSSTNESVKNLLLERVKESRTKPDSEAIKHLEQTMDEVRQILESKNEKGFLWQQINHISPNARQYCEQVKAALREHLEHNQVLSDTQKEEIRKAMDRMDNIVNDSSKDSQQKYREIRREVIELNAKATTPEDDNQLTRSHFQKAYFEGDTQKTLNLESLSQTLNQLSKAHYGETSMTDKITQRLDGYKNRNWFWNSVKEVLNFFNIPLPKLHSEVKEQIADKLKERLVDLKEKGMGNDVNAITRELGKAREDLIEHYKKTGELDKIINKSMEELLVARK

General information: TITO was launched using: RESULT: Template: 4M0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2531 188933 74.65 388.75 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 74.65 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.207

(partial model without unconserved sides chains): PDB file : Tito_4M0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M0M-query.scw PDB file : Tito_Scwrl_4M0M.pdb: