Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVRKALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDKIIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIYSYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTESRHKLEKFRFVDFAKRKK 3L5Z Chain:B ((7-149)) ------------------------------------------------------------------------------------------SEVESKIIEFTIVGADEIIAEKLGISVGDFVYKIIRLRIIHSIPTIMEHTWMPIAVIPGVEASVLEESIYSYIQNKLGLKVGTSVVRVKGIRPDDKEKQFMNLTNQDFLMRVEQVAYLTDGRTFEYSYADHLPETFEFETVIT---

General information: TITO was launched using: RESULT: Template: 3L5Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 617 -73895 -119.76 -516.74 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -119.76 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_3L5Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L5Z-query.scw PDB file : Tito_Scwrl_3L5Z.pdb: