Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSLLGDHLLQRLQQDTEGKLHQRLFRCLRGAIIDGVIQPKTRLPASRDLASEIHVSRNTVLTAYEQLQAEGYLEALTGHGTWVAEKLPESFLNTQNKKKVVSSPNVQSSYALSQRGSNLLGYAAASPHQWGAFVPGAPDVTEFPHHIFSRIQAR-----LSREPDINRLIYSNAGGCIELRSALADYLRVAR---SVQCDA----DQIIITEGIHQAIDLVSRALSDMGDKVWIEDPAYWGMRNTLRINGLDIQPMPVDAEGIIPEENPA---------------VPPKLIFVTPSHQYPLGSHLSLDRRRQLIQIARQHNSWIVEDDYDSEFRFSGQPYPSLQGLENNAPVLYMGTFSKTIYPSLRIGYLVVPKPLFSPLRIVAAELYRGGHLLEQKALAEFIRE---GHYEAHIRRMRLLYGKRRDYLVSLIQRYLGPEF-IHEYDEAAGLHLVLKLPNSCDDVAIA-ATALERGVKVRPLSQYYMQSHAHAERGLLMGFACVNEKDMVMAFGVLLQYLREADVPTLN 5TF5 Chain:A ((30-424)) -------------------------------------------------------------------------------------------------------------------------------PKSMISLAGGLPNPNMFPFKTAVITVENGKTIQFGEEMMKRALQYSPSAGIPELLSWLKQLQIKLHNPPTIHYPPSQGQMDLCVTSGSQQGLCKVFEMIINPGDNVLLDEPAYSGTLQSLHPLGCNIINVASDESGIVPDSLRDILSRWKPEDAKNPQKNTPKFLYTVPNGNNPTGNSLTSERKKEIYELARKYDFLIIEDDPYYFLQFNKFRVPTFLSMDVDGRVIRADSFSKIISSGLRIGFLTGPKPLIERVILHIQVSTLHPSTFNQLMISQLLHEWGEEGFMAHVDRVIDFYSNQKDAILAAADKWLT-GLAEWH-VPAAGMFLWIKVKGINDVKELIEEKAVKMGVLMLPGNAFYVDSSA-PSPYLRASFSSASPEQMDVAFQVLAQLIKES------

General information: TITO was launched using: RESULT: Template: 5TF5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1758 -29715 -16.90 -81.86 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -16.90 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_5TF5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TF5-query.scw PDB file : Tito_Scwrl_5TF5.pdb: