Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFLNPQYLFQLLSPCLLCEIGTREKYSLCKECWEQLPWLKQTIQRNDQSVLVACHYAYPINRIIQQFKYEQKLHYQTLLAEVLQQLK-FPKVQAIVPMPISKQRLTERGFNQSLLLANLLGKQLKIPVWQPVQRLNEHSQKGLSRLERFENIEQQFVALTQENRRYRRVLIIDDVITTGSSIHALSQALKQLGCTSIHASCLAAASSTSY 4M0K Chain:B ((58-179)) --------------------------------------------------------------------------PELLRLAIEALLEPFQEQEITKVVGIES-------RGF----ILGGMLAHHLDAGFVP-VRKKGKLPYQTLAESYQLEYGTDTIEMHIDAIEPGDRVLIHDDVIATGGTAEATIRLVERAGGEVVGCAFLIELT----

General information: TITO was launched using: RESULT: Template: 4M0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 595 -18445 -31.00 -152.44 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -31.00 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_4M0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M0K-query.scw PDB file : Tito_Scwrl_4M0K.pdb: