Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNPTDFQSSALATLRIEQQAIDVLATQIDD-RFNRACEILLQCKGRVVITGMGKSGHIGRKMAATFASTGTPSFFMHPGEAGHGDLGMLVRGDVLIAISNSGKSDEIMMLMPLIKHLGVPLITISRDDKGPMPQNADIALTLGESDEACPL----GLAPTSS--TTATLVLGDALAVALLEARGFTADDFARSHPAGALGKRLLLHVKHLMHTGEELPKVSPETPMNQVLYEISNKRLGVTTIVDEQDHLLGIFTDGDLRRLIDKQQGFDVNLPVSEVMTKKPSTISQEARAVEALQQLNQKKISQFVVVDDQNKVIGVISMHDLIQAGVN 1VIM Chain:D ((13-189)) ---------SLLRFLEVVSEHIKNLRNHIDLETVGEMIKLID-SARSIFVIGAGRSGYIAKAFAMRLMHLGYTVYVVGETV-----TPRITDQDVLVGISGSGETTSVVNISKKAKDIGSKLVAVTGKRDSSLAKMADVVMVVKGKMKQERDEILSQLAPLGTMFELTAMIFLDALVAEIMMQKHLTEKDLE--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 821 -36720 -44.73 -216.00 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -44.73 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_1VIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VIM-query.scw PDB file : Tito_Scwrl_1VIM.pdb: