Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIFLPGASGSTTFWHPLIEKLP-QQYRAKIIGYPSFGDTPESLEVKSFEDLTNYV---VNQI-HDESVIIAQSMGGIFAVTAALQKPQLVKGLVLIATSGGINLEP-----FN---VQDWR---------------EAYRQA---------------FLKY-------------PDWFITT----N--------ANYEEFLSDINIKTLLIWGDNDPVSPVQVGQYLNQKFENSTLYVVKGGDHQLAEKYADEVAVQIKNYLKGLM 3U1T Chain:B ((30-296)) QPVLFLHGNPTSSYLWRNIIPYVVAAGYRAVAPDLIGMGDSAKPDIEYRLQDHVAYMDGFIDALGLDDMVLVIHDWGSVIGMRHARLNPDRVAAVAFMEALVPPALPMPSYEAMGPQLGPLFRDLRTADVGEKMVLDGNFFVETILPEMGVVRSLSEAEMAAYRAPFPTRQSRLPTLQWPREVPIGGEPAFAEAEVLKNGEWLMASPIPKLLFHAEPGALAPKPVVDYLSENVPNLEVRFVGAGTHFLQEDHPHLIGQGIADWLRRN-

General information: TITO was launched using: RESULT: Template: 3U1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 950 -24277 -25.55 -121.99 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -25.55 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_3U1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U1T-query.scw PDB file : Tito_Scwrl_3U1T.pdb: