Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFDHIAQPISYQHIELS---F--PVHLDIKRLDLVHP-QISGNKFFKLKYNLLAAKEQGLSSILTFGGAYSNHIAATAYAAHLFGLKSIGIIRGEELAG------KPLNPTLAKAQSLGMQLHFVSRHEYRLRDE---ANYLKQLQQQFPQTYIIPEGGT-NELAVQGCQEILSQYDLE------QYDVICCAVGTGGTISGLIERSAA---HQKVLGFSALKGDFLQ-QEIKQ-------WTK----KQNWSLTDVYCWGGYAKTSPELFTFIENFEEQYTVPLEPIYTGKMMFGLFDLIKNNYFPANTRILAIHSGGLQADIRNRSHA 1J0C Chain:D ((10-335)) --PLTFGPSPISNLNRLSQHLGSKVNVYAKREDCNSGLAFGGNTLRKLEYIVPDIVEGDYTHLVSIGGRQSNQTRMVAALAAKLGKKCVLIQEDWVPIPEAEKDVYNRVGNIELSRIMGADVRVIEDGF-DIGMRKSFANALQELEDAGHKPYPIPAGCSEHKYGGLGFVGFADEVINQEVELGIKFDKIVVCCVTGSTTAGILAGMAQYGRQDDVIAIDASFTSEKTKEQTLRIANNTAKLIGVEHEFKDFTLDTRFAYPCYGVPNEGTIEAIRTCAEQEGVLTDPVYEGKSMQGLIALIKEDYFKPGANVLYVHLGGAPALSAYSSF-

General information: TITO was launched using: RESULT: Template: 1J0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1619 33606 20.76 116.28 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.70 3D Compatibility (PKB) : 20.76 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1J0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J0C-query.scw PDB file : Tito_Scwrl_1J0C.pdb: