Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNITD-QEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH 1SLH Chain:C ((36-133)) -----------------LPSRAHDGDAGVDLYSAEDVELAPGRRALVRTGVAVAVPFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGY------RGEIKVALINLDPAAPIVVHRGDRIAQ---------------------------

General information: TITO was launched using: RESULT: Template: 1SLH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 402 -42365 -105.38 -436.75 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -105.38 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_1SLH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SLH-query.scw PDB file : Tito_Scwrl_1SLH.pdb: