Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGNRANIEEIL-GNRVELVVGDIADAELVDKLAA--QADAIVHYAAESHNDNSLNDPSPFIHTNFIGTYTLLEAARK----------YDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSPYSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAADA-------YDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEK-----EAVEANYAKTQEIITV 1KEU Chain:A ((1-360)) ----MKILITGGAGFIGSAVVRHIIKN-TQDTVVNIDKLTYAGNLESLSDISESNRYNFEHADICDSAEITRIFEQYQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYALLEVARKYWSALGEDKKNNFRFHHISTDEVYGDLPHPDEV--ENSVTLPLFTETTAYAPSSPYSASKASSDHLVRAWRRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHARALHMVVTEGKAGETYNIGGHNEKKNLDVVFTICDLLDEIVPKATSYREQITYVADRPGHDRRYAIDAGKISRELGWKPLET-FESGIRKTVEWYLANTQWVNNVKSGAYQSWIEQNYEGR------

General information: TITO was launched using: RESULT: Template: 1KEU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1867 21235 11.37 63.39 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 11.37 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_1KEU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KEU-query.scw PDB file : Tito_Scwrl_1KEU.pdb: