Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAYGHGAVAVAKAI-QDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFTLTVAGLEWIQALLDK-EV-DLTGLTVHLKIDSGMGRIGFREAS-EVEQAQDLLQQ-HGVCVEGIFTHFATADEESDDYFNAQLERFKTILASMKEV-PELVHASNSATTLWHVETIFNAVRMGDAMYGLNPSGAVLD-LPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIATVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNGDKEITATQVATYRVTINYEVVCLLSDRIPREYY 4A3Q Chain:B ((2-369)) -SDKYYRSAYMNVDLNAVASNFKVFSTLH-PNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKD-TNTYQEVIEIIQQYEQLVFEGVFTHF----------TTEQYQRFKDMVNE--AIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTL----------------PTTIALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYH

General information: TITO was launched using: RESULT: Template: 4A3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1855 -46904 -25.28 -139.59 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -25.28 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_4A3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A3Q-query.scw PDB file : Tito_Scwrl_4A3Q.pdb: