Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKMKSPILRLRRLRRTKAIRGLLCENNFSVEDLVQPIFIEESITQPIPISTMPGIYRLPEQDIEQEVKALYALGIRCVMPFG---ISHHKDSMGSDTWSDTGLLVRMIKAIKSSCPEMIVTPDICFCEYTTHGHCGVMIHD-QISNDLTVENLIKQSLAAAKAGADMLAPSGMMDGQVGAIREALDQAGY-HNVLIMAHAIKFASALYGPFR-VAVDSNLVGDRHSYQLDYANTRQALREAALDESEGADLLIVKPGMFYLDILSQLRAQTK-LPLAAYQVGGEYAAIKFAAIAKALDERKTVIESITAFKRAGADVIITYFAKDIAIWHNEF 1H7R Chain:A ((27-340)) -------------------LRQWQSERQLTKNMLIFPLFISDNPDDFTEIDSLPNINRIGVNRLKDYLKPLVAKGLRSVILFGVPLIPGTKDPVGTAADDPAGPVIQGIKFIREYFPELYIICDVCLCEYTSHGHCGVLYDDGTINRERSVSRLAAVAVNYAKAGAHCVAPSDMIDGRIRDIKRGLINANLAHKTFVLSYAAKFSGNLYGPFRDAACSAPSNGDRKCYQLPPAGRGLARRALERDMSEGADGIIVKPSTFYLDIMRDASEICKDLPICAYHVSGEYAMLHAAAEKGVVDLKTIAFESHQGFLRAGARLIITYLAPEFLDWLDE-

General information: TITO was launched using: RESULT: Template: 1H7R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1755 -107522 -61.27 -350.23 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -61.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1H7R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H7R-query.scw PDB file : Tito_Scwrl_1H7R.pdb: