Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIPQSLLEQSIQINIQQALQEDIGDGDITAMLTPEDEQATATIISREDMVLAGQPWVNALISAYDNTVQVTWLKQEGDRVAANEAFLKLAGSARSLLTVERPALNFIQTLSAVATKTAEYVQHLEGLNTKLLDTRKTLPGLRIAQKYAVTVGGGQNHRLGLFDAFLIKENHIMAAGGIAQAIAKAHQIAP-GKPVEVEVETWDELNQALEAGADIVMLDNFS--QQQMIDAVK--HVAGRCKLEASGNITIENLREVATTGVDYISMGVLTKDVKAVDLSMRFNA 2B7Q Chain:C ((3-271)) -----------IRTFLERALKEDLGHGDLFERVLEKDFKATAFVRAKQEGVFSGEKYALELLEM--TGIECVQTIKDKERFKPKDALMEIRGDFSMLLKVERTLLNLLQHSSGIATLTSRFVEALNSHKVRLLDTRKTRPLLRIFEKYSVLNGGASNHRLGLDDALMLKDTHLRHVKDLKSFLTHARKNLPFTAKIEIECESFEEAKNAMNAGADIVMCDNLSVLETKEIAAYRDAHYPF-VLLEASGNISLESINAYAKSGVDAISVGALIHQATFIDMHMK---

General information: TITO was launched using: RESULT: Template: 2B7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1247 -71096 -57.01 -269.30 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -57.01 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_2B7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B7Q-query.scw PDB file : Tito_Scwrl_2B7Q.pdb: