Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENDMKKISLVIAASTMSLSVFAAAPITNKSPAKDQFSYSYGYLMGRNNTDALTDLNLDIFYQGLQEGAQNKTARLTDEEMAKAINDYKKTLEAKQLVEFQKQGQQNAQAGAAFLAENTKKSGVITTKSGLQYQVLKEGTGKT-PKATSRVKVNYEGRLLDGTVFDSSIARNHPVDFQLN--QVIAGWTKGLQTMKEGGKTRFFIPAKLAYGEVGAGDSIGPNSTLIFDIELLQVLPK 1FKI Chain:A ((1-106)) ---------------------------------------------------------------------------------------------------------------------------------GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKL---

General information: TITO was launched using: RESULT: Template: 1FKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 473 12608 26.66 122.41 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 26.66 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_1FKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FKI-query.scw PDB file : Tito_Scwrl_1FKI.pdb: