Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRIKVLPHAQFCPEGAEFEVEQNANLCQSLLNKGI-KIEHACDMSCACTTCHVIVRKGF-DSLEEMNDVEADLLDRAWG-LEPDSRLSCQVKVVDE--DLEIEIPKYTINHASENH 2M56 Chain:B ((14-102)) -----------------ELDVADGVSLMQAAVSNGIYDIVGDCGGSASCATCHVYVNEAFTDKVPAANEREIGMLESVTAELKPNSRLCCQIIMTPELDGIVVDVP-----------

General information: TITO was launched using: RESULT: Template: 2M56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 340 -8509 -25.03 -101.29 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -25.03 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2M56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M56-query.scw PDB file : Tito_Scwrl_2M56.pdb: