Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKRTFLFTIIAATLSTVYAAPLTKDNGAPVGDNQNSTTAGANGATLLQDVQLIQKLQRFGRERIPERVVHARGTGVYGEFVSTKDLSDLTLASLFK-AGTKTPVFVRFSTVIHPKGSPETARDPHGFAVKFYTQQGNWDLVGNNLPVFFIRDAIKFPDFVHAMKPDPVTNVQDPNRIFDFLQSQPWSINMLTYVYSNLGTAESYRTLDGFGVHAFKLYNDKGEYKYVKFNWRSQQGVKGLNLEQVREVQGRDWSHLTNDMYKNIYAGNYPKWDLYIQVLDPKDLDKFDFNPLDATKIWPNELVPEFKVGTLTLNRMPKNFFQETEQSAFAPGNLIPGIEPSEDRLLQGRVFSYSDTQLYRLGVNYQQIPVNRP-RVAVNNNNQEGFMNMGQTESHV-NYEPSQIEPKPATEKARAVQTPLEGTVMQHAIQKQQPYKQAGDLY-RSYSALEKKDLIRNLAADLGQVKNVETKTVMLSYFYKADADYGTRLAKAAQLKDE 7CAT Chain:B ((26-492)) ----------------------LTTGGGNPVGDKLNSLTVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITRYSKAKVFEHIGKRTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDALLFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMDGYGSHTFKLVNADGEAVYCKFHYKTDQGIKNLSVEDAARLAHEDPDYGLRDLFNAIATGNYPSWTLYIQVMTFSEAEIFPFNPFDLTKVWPHGDYPLIPVGKLVLNRNPVNYFAEVEQLAFDPSNMPPGIEPSPDKMLQGRLFAYPDTHRHRLGPNYLQIPVNCPYRARVANYQRDGPMCMMDNQGGAPNYYPNSFSAPEHQPSALEHRTHFSGDVQRFNSANDDNVTQVRTFYLKVLNEEQRKRLCENIAGHLKDAQLFIQKKAVKN-FSDVHPEYGSRI---------

General information: TITO was launched using: RESULT: Template: 7CAT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2239 10888 4.86 23.52 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 4.86 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_7CAT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-7CAT-query.scw PDB file : Tito_Scwrl_7CAT.pdb: