Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAALLGVNIDHVATLRQARGTAYPDPVEAALICEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLEMAVTDEMVEFAKEIQPQHVCFVPERRQEVTTEGGLDVVGNFEKVKAATQALAAIGCDVSLFIDADFAQIDAAIACGAPTIELHTGAYTDATTE----Q----------------E---QQEELARIVKGVEYAASKGLVVNAGHGLNLKNIAPIAAIPQIHALNIGHSIIAESVFVGLVQAVKDMKTAIQAAG 3O6D Chain:A ((2-260)) NAMLLGVNIDHIAVLRQARMVNDPDLLEAAFIVARHG-DQITLHVREDRRHAQDFDLENIIKFCKSPVNLECALNDEILNLALKLKPHRVTLVPEKREELTTEGGLCLNHA--KLKQSIEKLQNANIEVSLFINPSLEDIEKSKILKAQFIELHTGHYANLHNALFSNISHTAFALKELDQDKKTLQAQFEKELQNLELCAKKGLELGLKVAAGHGLNYKNVKPVVKIKEICELNIGQSIVARSVFTGLQNAILEMKELIKR--

General information: TITO was launched using: RESULT: Template: 3O6D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1238 -82826 -66.90 -350.96 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -66.90 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3O6D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O6D-query.scw PDB file : Tito_Scwrl_3O6D.pdb: