Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSTSVALIASVLRQPRVGDDLQWVRY-DAPFRTAQDYAAAEYLYRL--RLCSLRLRSSLLSPSLPRTGPVSEVAHLLPHYPVQITAQLVYMDEPLSPVVESAAVYASAAALLPPASPVGGDTDTHAEMEVVYTFTDEWLVFPLELCDVPLDASCKLHLWH----------RDTHVAE-----AALH--VYSVAGELCVGQRQLALSTGGDAPSDEQLWS-TSTRAAELLADFHRGMMPPIPWLDTLSIQQLEAEHAR-HGNAVVRRGSPQSAKDPCGAPTAILTLYLPAATTAVFFEPGVARVSDDMKSLLQHSDAADDSCDFTQRPFPDQYTFFKEHNLCEAKAAITSKTQYFLSDSSAPP-------GPKERHQLASLLRRPPIQLDNVAGAVV-----TGAGAGGVGGGRLEETRLLWKYRHFICR-DGKYFLPFMRCVDWANTHSSERRAACALIYQWARPAFEDVLACLSFYFDHVAPVRQYAVRLLRREGD-GRLCQLAFQLVQAVRY--DSAEAELANFLVERAVGCWELCSTLYTLLSVEVALEKR----RTLSAAGK-ASDDRHGGRALFEPLLRRLSERLTQQCPHFATRLRQQHAMHRVLQLLSRQLQQSSLDRLGKTALGNKLIAKQACGLRALFSSVHHGMPSRRNVNGSFSSSLMTHSSAGMREEHSMSSARTGDGSEEADANGGDGSSAVEASDVDSAADDEVRRLRFPPLSPQSQGSAPRSPPSQSGAKQQYRSRHASAVDVLDRYGVATLATHPGIPITGLLS--DSLYIFKSAKLPMRLTFTAL--RPAGLAWGGGRGGEPLYGASPSPCLLTPLAMQPAPSQQRSGGLQGMNSSGGGASAAVAAEEQAEGFLGAGEGDTVPLAMMYKYNDDIRQDQLIVQLIRLMDDLLQRDGLQLYLTPYRVIATAPNEGLVEIVPQVTTFFSVQR-----DVLKYLRVYNSTAELLRQAMDRYTRSFAGYCVITFVLGIGDRHLENILITQ-DGRLLHIDFG----YVLGNDPKPFPPPMKINREMVEVLGGPQSTGFTEFKLYCCSAYNTLRKHAPLLLHILLLGAHTEGMPQ-VTGEGGDPRVNLLKVQEKLRLDLTNAQATQYLQNVIADSVGSIFTNLWDVLHAAAQATRG----- 4JSP Chain:A ((10-1174)) ------------ERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLH-EWEDALVAYDKKMDTNKDDPELMLGRMRCLEAL---GEWGQLHQQCCEKWTLVND--ETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGK-SGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNN---LQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVA-------ILWHEMWHEGLEEASRLYFGER----NVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQA----WDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELA------------------VPGTYD-------PNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRY---------AVIPLSTNSGLIGWVPHCDT--------------------LHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDG--NYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATS-HENLCQCYIGWCPFW

General information: TITO was launched using: RESULT: Template: 4JSP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4502 48544 10.78 48.79 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 10.78 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_4JSP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JSP-query.scw PDB file : Tito_Scwrl_4JSP.pdb: