Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFQLKTLSVFLSALGAFSYSSVAQAQLMFSQYVDGSSNKKGLEIYNPDGTTVNLADYEIQQFNNGGTAKTATFRLQGTLASEQKFLVGRSELQTELGNKVNQVAALSFNGDDAVVLVYKGTPVDRFGRIGERPETGWGTAVSSLGNSFKRIETENPALSIDPTAAFDLDHSWTAWANRNDFTNLSGSTTQPPIETVSCSSSDTPIADLATSAQNQTYTVRGVITADYRYANGFSGFYVQTPDTKARANVSNAIFVYIPNSSTVKGGQIGDEVILRGRLTSYQNQLQIDQLQQDIQTCNSNMANQVQPISLELPFSSLTGGSTHSPQRYQGMLVKLPQTLMVSENYNYGRYGELSLSLGRLYIPTNLYPALSPEAKALAQKNLLSKIIFDDGYNNQNRTPWLPTNFSAANTLRSGYQLKNVEGILEYRFNGWRVQPVLGRTQPEVIAQTNSRQNVIAKNANHIRVASFNVLNYDNGATGFPTERGANTQAEFDKQHRKIVSALKSI-DADVYGLMEIANNGYGPNS------A----IAHLTSALG------PDWKYVIPENLDRLGTDVIAVAIIYNSKRVKPLNKAVVLDLGEKNRTTLAQTFQAIRGNKTFTVIPNHLKSKGCSGVDANSSDADQNDGQGCWNPTRVKAVDQIVQWLAKNPT-QVPKQNALLVGDMNSYAKEAPILAFEKANYKVLLNDTKVGQGAQAYSYVFGVASDANGNGGAGNLDHAIADADLYPKVVRTFAWHINADEPTVLDYNEEYKTDEQKALFYGEDAYRSSDHDPVIVDLDLNGKDSNQPNDNQKSPIFDFLSQLMEWISQLFKRS 2F1N Chain:A ((1-251)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLTDFRVATWNLQGASATT--------------ESKWNINVRQLISGENAVDILAVQEAGSPPSTAVDTGRVIPSPGIPVRELIWNLSTNSRPQQVYIYFSAV---DALGGRVNLALVSNRRADEVF---VLSPVRQGGRPLLGIRIG------NDAFFTAHAIAMRN--------------------NDAPALVEEVYNFFRDSRDPVHQALNWMILGDFNREPADLEMN-LTV-P-V--RRAS-EIISPAAATQT---------S--QRTLDYAVAGNSVAFRPS---PLQAGI-------VY------------GARRTQISSDHFPVGVSRR-----------------------------------

General information: TITO was launched using: RESULT: Template: 2F1N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1317 -1279 -0.97 -5.49 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -0.97 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_2F1N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F1N-query.scw PDB file : Tito_Scwrl_2F1N.pdb: