Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQITPYEVIDGQLKGARQVPSPNFNQRPAGTEIQMIVVHNISLPPSQFGGGYIEQFFQNKLDWSVHPYFQTIEGMQVSTHLLILRT--GEVLQFVNFNDRAWHAGRSSYLAKIECNDYSIGIELEGSDD---------LPFEDLQYEVLTDVVTAIRQAYPEIKNHIAGHSDIAPGRKTDPGPYFKWQHFRQLLAQKKT 4BJ4 Chain:B ((12-163)) ----------------------TDYTSANQDSRVQFIVLHYTSTDLPHS----L-------------GIL---THGGVSAHYLIGDDEPATVYRLVDENRRAWHAGVSEWQGRTWLNATSIGIEIVNQGYRDTPQGRVWYPFSEAQIQALIPLLKDIAKRHGITPDRIIGHSDIAPGRKVDPGPLFPWKRLADA------

General information: TITO was launched using: RESULT: Template: 4BJ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 626 -40547 -64.77 -287.57 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -64.77 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_4BJ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BJ4-query.scw PDB file : Tito_Scwrl_4BJ4.pdb: