Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYRLSMSLIANTTVVRGRFLDIQQTVSEPTDIPNQVRYLEDGVLISEHGKIKWFGAWEDAQQHLPAGVEVQHYPEQLIVPGFIDTHIHFPQTEMVGAYG-EQLLSWLNTYTFPTEIQFQDKTYASEIAQFFVQELLKHGTTTALVFCTVHPESVDALFEAAERVQMRLIAGKVLMDRNAPEALCDTPETAYSNTKALIEKWHG-KGRALYAITPRFAPTSTPEQLERAGQLKQEFPDVYVHTHLSENKDEIAWVKSLFPEQAGYLDVYQHYGLTGKRSVFAHCVHLEDEEWQCMHDTQSAIAFCPTSNLFLGSGLFPLKKTWEKQVKVGLGTDIGAGT-SFSLLQTVNEAYKVQQLQ-GDKLSAYEALYHATLGGAKALDLQDQLGNFNIGKEADFVVLNLKPTALQELRQTKSKSVEDSLFALFTLGDDRNIEATYIYGNRAYQKETAK 1P1M Chain:A ((15-384)) ---------------------------------------EPFWGAVEIENGTIKRVLQG--------EVKVDLDLSGKLVMPALFNTHTHAPMTLLRGVAEDLSFEEWLFSKVLPIEDRL-TEKMAYYGTILAQMEMARHGIAGFVDMYFHE----EWIAKAVRDFGMRALLTRGLVDSNGD------DGGRLEENLKLYNEWNGFEGRIFVGFGPHSPYLCSEEYLKRVFDTAKSL-NAPVTIHLYETSKEEY-----------DLEDILNIGLKEVKTIAAHCVHLPERYFGVLKDIPFFVSHNPASNLKLGNGIAPVQRMIEHGMKVTLGTDGAASNNSLNLFFEMRLASLLQKAQNPRNLDVNTCLKMVTYDGAQAMGF--KSGKIEEGWNADLVVIDLDLPEMF--------PVQNIKNHLVHA-FSGEVFATMVAGKWIYFDGEY-

General information: TITO was launched using: RESULT: Template: 1P1M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2218 -69213 -31.20 -189.11 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -31.20 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_1P1M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P1M-query.scw PDB file : Tito_Scwrl_1P1M.pdb: