Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAWTPHVTVATVVEKDGRYLFVE--EHSEGFVHTVFNQPAGHVECGETLTEAAIRETLEETGHHIDIDALLGIYTYTPPMFPDRTYYRFCFLAHVTHVESDPKLDTGIVSAVWMTLDELKESARARSPLVIKAIEDAMKGQHYPLALIYEHPFSPSLTSHLDA 5IW5 Chain:B ((129-232)) ----APCIIVA--IRRDDSILLAQHTRHRNG-VHTVL---AGFVEVGETLEQAVAREVMEESG--IKVKNLRYV-TSQPWPFPQSLMTAFMAEYDSGDIVIDPK---ELLEANWYRYDDL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IW5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 448 -44891 -100.20 -440.10 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -100.20 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_5IW5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IW5-query.scw PDB file : Tito_Scwrl_5IW5.pdb: