Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIENIGGMNMVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHELGVEMAIVVGGGNIWRGQI-GAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQTSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILMAKN-NVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNLNEHGNIRRAILGENIGTTVRGK 1Z9D Chain:B ((4-240)) ------------EPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSGVQIALVIGGGNLWRGEPAAD-AGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQTAIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA-KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMNEAGNIQRVVFGEHIGTTVSN-

General information: TITO was launched using: RESULT: Template: 1Z9D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1264 -66490 -52.60 -282.93 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.89 3D Compatibility (PKB) : -52.60 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_1Z9D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z9D-query.scw PDB file : Tito_Scwrl_1Z9D.pdb: