Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLVKEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAY---GNNASKCFGVVGSGNRN-FNNQYCLTAKQYSQRFG-FPVLA---------------------DFEMRGML--E-DIKHVAAIIADLYELEKEN 1FUE Chain:A ((1-162)) -GKIGIFFGTDSGNAEAIAEKISKAIGN------AEVVDVAKAS---KEQFNGFTKVILVAPTAGAG----------DLQTDWEDFLGTLEASDFANKTIGLVGLGDQDTYSETFAEGIFHIYEKAKAGKVVGQTSTDGYHFAASKAVEGGKFVGLVIDEDNQDDLTDERIAKWVEQVRGSF---

General information: TITO was launched using: RESULT: Template: 1FUE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 659 -43677 -66.28 -328.40 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -66.28 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_1FUE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FUE-query.scw PDB file : Tito_Scwrl_1FUE.pdb: