Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRVLITGVSSGIGLAQA-RLFLEKGYQVYGVDQGE---KPLLE------GDFRFLQRDLTLDLEPI------FDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVELTRYYLTQMLENK-KGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGIQVFGIAPGAVKTAMTAADF-EPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAMQGQILTIDGGWSLK 1RWB Chain:E ((8-252)) --KVVVITGSSTGLGKSMAIRFATEKAKVVVNYRSKEDEANSVLEEIKKVGGEAIAVKGDVTVESDVINLVQSAIKEFGKLDVMINNAG-LENPVSSHEMSLSDWNKVIDTNLTGAFLGSREAIKYFVENDIKGTVINMSSVHEKIPWPLFVHYAASKGGMKLMTKTLALEYAPKGIRVNNIGPGAINTPINAEKFADPEQRAD-VESMIPMGYIGEPEEIAAVAAWLASSEASYVTGITLFADGGMTL-

General information: TITO was launched using: RESULT: Template: 1RWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1212 -18457 -15.23 -81.31 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain E : 0.77 3D Compatibility (PKB) : -15.23 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_1RWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RWB-query.scw PDB file : Tito_Scwrl_1RWB.pdb: