Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNG-KANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSK-PWQTLWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANK--INDADVIITYGDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK 4MDY Chain:A ((11-293)) -----------------------------------DDGSVTVRHAFGDTTIPGPPQRVVSAGLTEQDDLLAVGVVPIAVTDWFGGE--PFGVWPWAQ---RQLAGAQPAVLNLDNGIPVEEIAALKPDLIVATNAGLDADTYAKLSEIAPTVAQTGSEAFFEPWKDQATIIGQAVFKNAEMTELIKSVDDRFTTVKTDHPQFS----GKKALLLGGTL--YRGGVQATPPGWRTDFLTQMGLTVLQV----------PALIPRDEIASVLDGADVLIWTTESDQDRDALLADPIVAQLAATRRDRNIFTTKEL-AGAIAFASPLSYPVVADQLPPELARV-----

General information: TITO was launched using: RESULT: Template: 4MDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1533 11474 7.48 41.13 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 7.48 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_4MDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MDY-query.scw PDB file : Tito_Scwrl_4MDY.pdb: