Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFSPLCWIVPSIYVIMKEGFKIGKGVFMLKLGVIGTGAISH-HFIEAAHTSGEYQLVAIYSRKLETAATFASRYQNIQLFDQLEVFFKS-SFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEWFDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLAD---KQVLGADFNYAKYSSKMPDLLAGQTPNVFSDRFAGGALMDLGIYPLYAAVRLFGK-ANDATYHAQQLDNSIDLNGDGILFYPD-YQVHIKAGKNIT-SNLPCEIYTTDGTL-TLNTIEHIRSAIFTDHQ------GNQ-VQLP-----IQQAPHTMTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK 3M2T Chain:B ((2-336)) -------------------------SLSLIKVGLVGIGAQMQENLLPSLLQMQDIRIVAACDSDLERARRVHRFISDIPVLDNVPAMLNQVPLDAVVMAGPPQLHFEMGLLAMSKGVNVFVEKPPCATLEELETLIDAARRSDVVSGVGMNFKFARPVRQLREMTQVDEFGETLHIQLNHYANKP-------RAPLWGLDST-LRSFLLAQAIHTIDLAITFGDGELRRVQSSVQR--HDDALIVRADMAFSSGATASLLAGTSFPYFEFDMKLVSSSSTLVELDNLWNI-TLHEPEHATRPTGAKRWRGAWQPGPLDSGYERSGYHGELHQFFQAIREHR--RFEADFASLLPTYRVIEEICSADAVAQGL--

General information: TITO was launched using: RESULT: Template: 3M2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1645 -26263 -15.97 -83.91 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -15.97 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_3M2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M2T-query.scw PDB file : Tito_Scwrl_3M2T.pdb: