Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDYTEKQVLQGKAVLFLGAGASYNCTDREKKRIGFTGNELLQKINEEFLGNTPNHLTLDFASTMAIQKAGRENFDLFIKELVCDFDPTNEHKLLSEFKWKAIFTTNYDEAIEKAYRENKSALQKIEKIIS----DNDSLQKVVVNPDKLPII--KIHGCISRPNDTRTPLVISCSDYRRHQENRSSLYQYLKECLTSDLVIFYGYGLGDNNIVGILDDLEKEGVNRTRHIWLDPFMDEMYKDYWESKNLLCKVNNLYDFLSEIKSKKDSNILSLNSFLKNDSVISKLIPSNDRPSNELETYLLKQLLYVEMNDEIKRESDNYNNDIFYRGNAHGFSWVAKNLDFQRAIERTLEVELFENFEISNQLFNFLIINGYAGSGKSVLLKRLAWNGVSKFNGNCFYLSEGATLNTKLIFELIKLIKEPIILFVDNILEHQTEILEIKNFSIKDCSKIM---IVGAARSNEWNNSENILDKLNPNFFSLKDL-NDFEVKNLVNKLKEFKAEGSLKELSDIDKYKFIKEKSNNQLLVTLLEATHYGQEFSEIIKDEYEGIYDRSAKDLYLSICCFHRHGLELRAGMIKRLSGINFEQFKEKFLQPLELLVTSYYSYRVNDIVYTTRHSYIAQHVFKQAFLSEMDKAQQLIKII--RYLNIGFDTDSKALDFILKGRILAEEFNDKDLAHSIFNIAQDIGVNNAFILHQRAIFE-INHQNPNYELALDYLKRINSSDGYYDMRTVEHTKANAYRKIALNSSSKQDKIKYRNLSLKLLNDNIRTSNRTSMPYHTKGLVLLDEIKDCDNENDLVDIIKDFENNLSAGFKKFPYDESLTVLEHDFSDEIDNSPRAIEKIKEALRKNSDNIYIVQRYAKFYIKKNDFLEARTCMLNFLRNDVNNRAINYLMAYSYIKENESLYFQNAIKYLKKCYSPRDSNYEPKFLHACLEYIYQSEEKALKIFTDLNQSNVSPKIKNKPTHMIRDSEGNERFFDAIIVTINDQFGFVKTSKYAENIYVHKSTISNEEDW-EALRP--GDPLKISIWFSFRGPRAKTAIIL-- 5F56 Chain:A ((2-704)) --SRPAHWLLAPPA----------------------SRDALLATMREWQV------------SPPVAQVLCGRDLRTELLALPLELTP--------------------NPALREAARHIVAAVREGKRIRIHGDYDADGVSATATLVLGLRAIGANVHGFI--PHRLNEGYGIHPDRVPEHAAA-------------ADLVVTVDCGVSN------LDEVKSLLATGTEVVVTDHHAPG------------------------------------ENFPECLVVHPHLTPDYDPDRHNLTGAGVAYHLLWAVYEELGRPE-------------------------------------------PRALLPLATLGTVAD--------------VAPLLG----------ENRALVRAGLAEMARTELPGLRALMNEK----RVRQPTARDVAFILAPRINAAGRMGEADRALELLT--TPSDHEAKSLAAYLEIRNQERRKIQDDMFAQALQLAD----------PNDPALVLTHDDWH---------------------------------------AGVM----GIVASKLVETFNRPVYIVAQGKGSVRSTPGI---------------SAVQGLRESRDLLGRFGGHPGAAGFSLDPQNFGALRERIHGYVRQFPTPVPAVRLDAPLPVAALTPELLSELSILEPFGEGNPRPL----WHLRGPLTDTRLVGKQGDVLQFRFGGVKGMKYSERDDAAGERDVAAELALNEWK--GRTSLELHAAALRPLAPL------------------------------------------ALAGTEEGLPTLP---RLN-------PREAMTFLKTGAAAYAEQGVATYLRDNVPG--------LTLLDTNAPHPGGDLILY-----------GLPP-ESALRRWLHEAQEQGGRVAFALGPKTLAELDAALTLAKLLPD------------SHTEAAQEAAADAYRSWQWAHHYRVLNDAGWSASVYAMLGLPVPAAL------PKAAEALALAA

General information: TITO was launched using: RESULT: Template: 5F56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3934 140166 35.63 204.62 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 35.63 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_5F56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F56-query.scw PDB file : Tito_Scwrl_5F56.pdb: