Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLYATQDEKKQAAAKAALKHLPKGGILGVGTGSTVNFLIDLLPEL--QLEAAVASSQATADRLKKLGIEVVDMNHVVSLDAYVDGADEIDRHMHMIKGGGAALTREKIVASIAKKFVCIVDDSKWVDQLGRDFPLPVEVIPMARSAVARKLVSLGGDPVYREGVVTDNGNVILDVFNLNILNAIDLEKTINNIPGVVTNGIFALNPATI-AIVATNDGIEERTAQ 5UF2 Chain:A ((9-227)) ---MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSGKKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHLNEKIVASASEKFVCIADESKYVSRLG-KFPLPVEAVESARSLVSRKLLAMGGQPELRIGYTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFAR-DAADVLILGTEEGAKVIY--

General information: TITO was launched using: RESULT: Template: 5UF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1276 12789 10.02 59.21 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 10.02 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_5UF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UF2-query.scw PDB file : Tito_Scwrl_5UF2.pdb: