Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILIVHAHPEPLSFTTSLKTTAQQTFKKLGHQVVISDLYAMQFNPLASKEDFL-ELNQPDYFNYALEQRNATKQQLLSPDIQAEIDKVKRADLIILNFPLYWTSVPAILKGWIDRVFVSGLFYGGKRFYNHGGMAGKKAMLCLTLGGRTHMFGENSIHGPIEHYLSPIQRGTLAYTGFEVLPPFIAYHVPYISQEARETILEDYVTYLTHLDQLEPLKFPKLEEFDEKLYPL 2QX9 Chain:B ((4-220)) -KVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPI---------------

General information: TITO was launched using: RESULT: Template: 2QX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 999 -85185 -85.27 -394.38 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -85.27 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2QX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QX9-query.scw PDB file : Tito_Scwrl_2QX9.pdb: