Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQVKLLDPRLGKEWPLPSYATAGSAGLDLRACLDEAIEIEPGQTVLVKTGMAIYIHDVNFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGQTTFRLEPGERLAQYVLVPVVQAEFEQVEEFEETLRGAGGFGHTGKQ 2OL0 Chain:A ((22-131)) ------------------PTRQSPGAAGYDLYSAYDYTIP--PGERQLIKTDISMSMPKFCY-GRIAPRSGLSLK-GIDIGG--GVIDEDYRGNIGVILINNGKCTFNVNTGDRIAQLIYQRIYYPELEEVQSL----------------

General information: TITO was launched using: RESULT: Template: 2OL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 440 -68004 -154.55 -618.21 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -154.55 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_2OL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OL0-query.scw PDB file : Tito_Scwrl_2OL0.pdb: