Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGF------KVKNVDNYAGHIDLGEGDETLGIFGHMDVVPA-------GDGWDTDPYEPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMAYYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVG----KGAHGASPQSGINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGE-KLGVAYEDEKMGKLTMNAGLFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVPMD-DPLVATLLQVYEDHTGEK----GYEQI---IGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK--- 5K8P Chain:A ((5-425)) NDVAKVMKTLDGMREGLIQTAVELGSI-----------EAPTGRE-GAAGDYVYEWMARNGFGPERVGVFDDRFNVVGRLRGTGGGASLSFNSHLDTIMAREDTARFADANDRIYHEAWHEEGRIYGYSVVNCKGPMACWLIAAKALKEAGAALKGDVVLTAVCGEI------------DCEP------------VDEFQGHDYLAEDI---------------GARYAISHG--------AISDYALVAEATNFK-------PAWVEAGKVFLKVTVFAGPSRYTPYVPRPVAALDSPNAIVRMAKLVEALEEWADNYEKR--YTREYGGGTVVPKVAIGAIRGGV--------PYKIYAFPELCSIYMDIRLNPDTNPLVVQREVEAVVSKLGLKAEVKPFLFRRGYEAQGIEPLQNALEVAHREVVGRPTERPGSPECSMWRDTNPYNELGIPSLTYGC-----GGGAGGGNTYFLVDDMLKAAKVYAMTAMDLCNRTP

General information: TITO was launched using: RESULT: Template: 5K8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2221 64996 29.26 166.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 29.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_5K8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K8P-query.scw PDB file : Tito_Scwrl_5K8P.pdb: