Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSLVKLVGE--EAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGN-VSDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS 1DFH Chain:A ((2-253)) --FLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIA

General information: TITO was launched using: RESULT: Template: 1DFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1347 -84140 -62.46 -337.91 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -62.46 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_1DFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DFH-query.scw PDB file : Tito_Scwrl_1DFH.pdb: