Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSITLLQPDDWHAHLRDG-----LALKR-TVPDLAKQFARAICMPNLVPPVKTVEEALAYRERILAHVPEGNNFDPRMVLYFTDHTSP--DEVRKIKE-SEHVNAIKLYPAGATTNSNNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHNHV-----------------DIFDREKRFLDEVLSPLLKQF-----PKLKVVLEHITTSDAAHFVLEQD---RNVAATITPQHLLFNRNDMLVGGIKPYFYCLPILKRQTHQTTLLEVATSGNPKFFLGTDSAPHAQNAKEN--ACGCAGCYSAPNAIELYAQAF--DQVGKLERLEGFASHFGADFYGLPRNTSTITLVKEDNLVPESFDYLDNQKIIPLHAGKTLQWRKV 2Z00 Chain:A ((44-374)) -GCFLAPGFLDLHAHLREPGEEVKEDLFSGLLAAVRGGYTDLVSMPNTKPPVDTPEAVRALKEKAKALG--LARLHPAAA--LTEKQEGKTLTPAGLLREAG-AVLLTDDGRT--------NEDAGVLAAGLLMAAPLGLPVAVHAEDAGLRRNGVMNDGPLADLLGLPGNPPEAEAARIARD-LEVLRYALRRSPATPRLHVQHLSTKRGLELVREAKRAGLPVTAEATPHHLTLTEEALRT--FDPLFKVAPPLRGEEDREALLEGLLDGT-LDAIATDHAPHTLAEKEKDLLRAPFGIPSLEVAFPLLYTELHLKRGFPLQRLVELFTDGPRRVLGLPP--LHLEEGA-------------------------------

General information: TITO was launched using: RESULT: Template: 2Z00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1723 65815 38.20 224.62 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 38.20 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2Z00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z00-query.scw PDB file : Tito_Scwrl_2Z00.pdb: