Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSLNSSYGRSQAVPLSVFEQSKDAHIIRSDEEAIEIAKQLAQEFATEASDRDRERRLPLNEIQQFSQSGLWAITVPKQFGGAQVKYRTLAEVIKTIASVDSSLAQLPQNHLAFIEHLRLDAKPEQQQFFFNLVLRGI-RFGNAFSEKHSKTVADLTTTLRK-TEHGYEINGKKFFATGALLAHWIPIVAVNEL-----GQPYAALVPRETSGVNIINDWSGFGQRTTASGTVELNQVPVREEYLVPIYQAFES-PT------PAGAISQFIQAAVDAGIARGAIEETIQYVKNHSRAWIDSGLEKASDDPYTIANIGDLKIKLRAAEAVLDLAGDAIDQAIANTT---EEHVNQATLLVAEAKVLTTEIAILAANKLFELSGTRSTLSELNLDRHWRNARTHTLHDPVRWKYHIVGNYYLNGVQPPRHAWS 4DOY Chain:B ((38-434)) -------------------------------NDPVAVARGLAEKWRATAVERDRAGGSATAEREDLRASGLLSLLVPREYGGWGADWPTAIEVVREIAAADGSLGHLFGYHLTNAPMIELIGSQEQEEHLYTQIAQNNWWTGNASSENN-SHVLDWKVSATPTEDGGYVLNGTKHFCSGAKGSDLLFVFGVVQDDSPQQGAIIAAAIPTSRAGVTPNDDWAAIGMRQTDSGSTDFHNVKVEPDEVLGAPNAFVLAFIQSERGSLFAPIAQLIFANVYLGIAHGALDAAREYTRTQARPWTPAGIQQATEDPYTIRSYGEFTIALQGADAAAREAAHLLQTVWDKGDALTPEDRGELMVKVSGVKALATNAALNISSGVFEVIGARGTHPRYGFDRFWRNVRTHSLHDPVSYKIADVGKHTLNGQYPIPG---

General information: TITO was launched using: RESULT: Template: 4DOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2053 -18539 -9.03 -48.79 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -9.03 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_4DOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DOY-query.scw PDB file : Tito_Scwrl_4DOY.pdb: