Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFKNIADQTNGFYIPCVSLFGPGCAKEIGTKAQNLGAKKALIVTDEGLFK--FGVADLIASYLTEAGVASHIFPGAEPNPTDINVHNGVNAYNENGCDFIVSLGGGSSHDCAKGIGLVTAGGGHIRDYEGIDKSKVPMTPLIAVNTTAGTASEMTRFCIITNTDTHVKMAIVDWRCTPLIAIDDPKLMIAKPAGLTAATGMDALTHAVEAYVSTAANPITDACAEKAITMISQWLQPAVANGENIEARDAMSYAQYLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCNAILLPHVCEFNLIACPDRYAKIAELMGVNTHGLTVTEAAYAAIDAIRKLSSSIGIPSGLTELGVKTEDLAVMAENAQKDACMLTNPRKANHAQVVEIFKAAL 4FR2 Chain:A ((16-398)) ---------AYDFLMPSVNFFGPGVISKIGERAKMLGMKKPVIVTDKFLENLKNGAVAQTLASLKKSGVDYVVYNGVEPNPKIHNIKEVKTLYEKEDADSIITVGGGSAHDTGKGAGIIMTNGDDITKLAGIETLKNPLPPLIAVNTTAGTGSELTRHAVITNEETHLKFVVVSWRNIPLVSFNDPTLMLDIPKGLTAATGMDAFVQAVEPYVSVDHNPITDSQCIQAIKLIESSLREAVANGHNLQARTKMVEAEMLAGMAFNNANLGYVHAMAHQLGGQYDAPHGVCCALLLPYAEEYNLIADPERFAELARIMGENTDGLSTRDAAELSIKAMKQLSEDVGIPHSIKDIGAKPEDFDLMAENALKDGNAFSNPRKGTKEDIVKIFQEAY

General information: TITO was launched using: RESULT: Template: 4FR2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2333 -11854 -5.08 -31.11 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -5.08 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4FR2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FR2-query.scw PDB file : Tito_Scwrl_4FR2.pdb: