Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTSRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGTRVLKKVDAIVREEMNRSGAMEVFMPVTQPASLWEESGRYEQYGPGLLRFKDRHDNPFVLGPTHEEVITDLARNELKSYKQLPVNFYQIQTKFRDEIRPRFGVMRSREFIMKDAYSFHATQESLQETYNVMYDTYSRIFTRLGLDFRPVQADTGSIGGSASHEFHVLAASGEDDIAFSTESDYAANVEMAEAVLVGE-RAAPTQEFKLVETPNQKTIADVCQFLNADPKQSVKALLVQGVADEKGNVPVVALFLRGDHELNEIKAEKHPLVAAPLAFATEEQL-QAFGLTAGFTGPQGLVEKGITVIVDRAASVLSDFVAGANEADKHAVGVNWERDAQITEVFDLRNVVEGDPSPDGKGTLQIKCGIEVGHIFQLGTKYSEALGCKVLGEDGKPFTVTMGCYGIGVTRVVAAAIEQNYDDKGIIWPQAIAPFEIAIVPMNAHKSPRTLEAAEALYAELQAQGFDVLLDDRNERPGVKFSDLELMGIPHRIVIGEKGLDAGTFEYKGRRDAEASNLTKEELLAKLAR 2J3M Chain:A ((1-562)) MKQSKMLIPTL-------EVLSHQILLRAGYIRQVAAGIYSYLPLANRVLEKLKTIMREEFEKIDAVEMLMPALLPAELWKESGRYETYGPNLYRLKDRNDRDYILGPTHEETFTELIRDEINSYKRLPLNLYQIQTKYRDEKRSRSGLLRGREFIMKDGYSFHADEASLDQSYRDYEKAYSRIFERCGLEFRAIIGDG----GKDSKEFMAISEIGEDTICYSTESDYAANLEMATSLYTPKKSHETQLDLEKIATPEVGTIAEVANFFEVEPQRIIKSVLFIAD------EEPVMVLVRGDHDVNDVKLKNFLGAD-FLDEATEEDARRVLGAGFGSIGPVNVSE-DVKIYADLAVQDLANAIVGANEDGYHLTNVNPDRDFQPISYEDLRFVQEGDPSPDGNGVLAFTKGIEIGHIFKLGTRYSDAMGATVLDENGREKSVIMGCYGIGVSRLLSAIVEQNADERGINWPTGIAPFDLHVVQMNVKDE-YQTKLSQEVEAMMTEAGYEVLVDDRNERAGVKFADADLIGCPIRITVGKKAV-DGVVEVKIKRTGEMLEVRKEELESTLS-

General information: TITO was launched using: RESULT: Template: 2J3M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3317 -65518 -19.75 -119.34 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -19.75 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_2J3M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J3M-query.scw PDB file : Tito_Scwrl_2J3M.pdb: