Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAYYYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVARTFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKITDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDFFAFSGHKMC-GPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGTPNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTGVVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDADRLVEAIMATKEFFQHGTI 5B7U Chain:A ((21-417)) MRIPEDVRKDIPLTN------EVIYFDNTATSLTPKPVVEAMDEYYLKYRANVHRGVHRLSQMATHKYEESRKIVADFIGAK-FEEIVFTKNTSESLNLVALGLGHI-FKRGDKIVTTPYEHHSDLLPWQRLATKLGLKLEFIEGDDEGNLDLSDAEKKI-KGAKLVAVQHVSNALGVIHEVEELGKIAKDEGAIFVVDAAQSAGHMEVNVKKLHADFLAFSGHKGPMGPTGIGVLYIREEFFDTFEPPLIGGGTIEDVSLDGYKLTEPPERFEAGTPNIGGAIGLAAGIRYIERIGLGRIERQEHKLVKRTTEGLDEL-EVPWYGPRNLKKHAGVVSFNVPGLHPHDVAAILDDHSIMVRSGHH-ALPVMKKLGINGTVRASFHVYNSLEEVETFLGVMEELVKGL-----

General information: TITO was launched using: RESULT: Template: 5B7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2364 -118724 -50.22 -300.57 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -50.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_5B7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B7U-query.scw PDB file : Tito_Scwrl_5B7U.pdb: