Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSVDSDKLATSIRALAGSRDERIAFVTEGVKQAKELQGKSVQEYEMVVSSLLMCLESFEVVHGTSLDVLCELTVLDILLNLDLSVIQLSQCNSYLRLCLMAVNEIPVAVAAAKTFAQTLSYSMTTDFVRTQIGETLEWISLSDAKSQPRRICGILVLTEVALRIPMVILPRLSEVFDRAWDCLVVHDEEVRSRALCLFTLCAKLLVNRPAAIRAETCDALMSHLKGNLASKSKGKRIAGLLAFEPIVINSIGTSNIRYEDLSNLLVPYIMGGGANVNADTRELLFRCLVVLCRFSAPQFVAHQLKESVRFALDSINRNIQRNTAFEMLSEIIPIVGKTEFAPFVDDTCEVIKTIFEKSPSPCWKALQCFSIICKECRPSKMETYVESCIENVFVWGLSAELIECMRAIIESSSVQYRAKLEESLLDMISVTLCGLPFR-----------QQIDAPRVSESSTDVSASEYQISVALNALKQF-GFSNSELMGDFLRVSVLPFIDNNSVAVRNAAVYTIVKLLIPPGEKGDLSIARRMCVNTVITRMLVVGLSDPNPVVRQTILSAFTEDFYPYLSEQQYLFRFYSALGDESINCRVAATEQLCQMIRYDPSHILPALREEMVVILHFITSTFNMDTVQNGFRLLAAIATRAPQLVVNFTEGICEALAPYFSTLSSRSGILLSFLHCCTAVAAALRKFGSTPFSSKREIALVCELLETLPADRSYHIIRLSCLRFLSATLAPLMEGASPYVLYPRLFQQLCTVLHRTEESVDIRLEALRCIGVIGALDSHVFQSFNLNEKEKASGMSAVVVDRVTHVKCCRIVLCAVAALLDPESKRSAGAREGLLRTGVQTMLNIFEHVPCSKSEIGLVIGPLARVIRQLPGGRLFATVLFEFCNIIRYGGAVVLNDLNEIYRVFDNVWEFQVNYRFLAVRMLSFVANFEASGNLHYGQQHSRILPQLFDTLNRADLRADLKYAVLEYIVRHVDSLQPCTEAAVDNLLGAIQNPANPLDFVNHCVVTLKEICVKLYVDELVGAIVRCLLACLSVDLARKTRGRDNIALCNNIMGVFCVLIEELQGNFLKYSTYIIQALKNYRITNTEFGVMNNRVATGLRCARSAMS--IQQQNAEVAALLKTCESVVMKSMSRMGEVWSYANLNDDNANAGAAEERTMPINEQRLINPMKVVPL-TKEEWVKWSDQFAITLLQESPFQVFRCTAVPIGINAAPLVEKSTQFVQDTLQIAFRSMWNYASSALQTAITDFFRQSFRQAMMSTTVPAEVVTMLLGIVEYMDHVGEALFISYNDLSECAWNRGMLAKALFWREAAYRDDP-VGTVESLISLYSELHMVDSSVGILNMTSEEQRRSLLQTSLVKLARYTEALQLTQQELELEAAPLEESSTENLSKGCGYKNGSRGWSRMLHYTSNSDLTPSSSKAEESCRDRRVEVNARLMLCLSELGEYDKVLEQWGSMLHNYKDRVTDTDEMHVLFFVSQYAADASIRLQSWDTLEHVLDWLPPDMVLYHVSKAALEVVRGNYDAALVSVTNGRKVLLEDLTSLLHESYARAYEGLVVAQQLTEVEEVIIAKQTQKALSSAAHIPHLCHIWEQRIRMMHATVPTWKQVLGIRGLLISPHEDVKTRIQFTKLCRQEKARQLEKFTLAELLGFANPTLEQLTSRNVNPRVVMQYISYLSETNALGPGSA--FGTESDLIKKMIDVHTKAENSAVLARAYTRLGSKVDLIESVQCFKTAIMYDPQWFLAWRKWA----EANAQLLQTDKGEET------------------CRNAIEGFIRSIQLGTSDSTLIQDVLKLLTLWSSHCDSDHNLKELRERVFDVPSRVWHLVVPQLVARLDTGSDDSCRLVADVLTNVGYDYPHTLVYPLNLCTMSDSERRNRYSNEVLGKLQERYPVIVLQGRLMIDELIRVSALIYEQWYDKLEAAATAFFGRRSTDEMIRTLLPIHETLTRVPETVVESEFLSKYSKRLTEARDWLRSYSSTRYTGDIQSAWKLYHSVYCNIDKQIKSSDTLQLQFCSPKLFEARNLSIGIPDARPVREESVAKVNRFQKDIIVIASKQRPKRIGIITAEGRLQKFLLKGREDLRLDERVMQLFSLVNILMQSDSRTSKNLGFQIQRYSVTPLKDNVGIIGWVDGCDTLHEVVKHFRERKSIPVELEMRMLDQIITFDNSKAYDYLTIMSKVEVLEFLSDHTSGQDIRKAMWNTSPNCEVWYDRRRMYTTSLANMSIVGYILGLGDRHPNNIMLQRASGLVVHIDFGDCFEVAMTRDKFPEKVPFRLTRMLRSALDVSGVDGAFRACSETAMCVLREGSHSVLALLEAFIQDPLISWRLINRHGQEPESSNDHKTIEQQVNIANMRNATSTAGAESMCDSVGKRDSGTFSVAKHLCGEEVDVVHQGVFIVKRLSSKLKGQDFISAGTEPLDPRTQVGQLIEEATDVTNVAQSWSGWYPFW 5FVM Chain:A ((85-2469)) ---------NVKAFDLIFTKLNQLERVAASYELKSCLISLVSTEYFQRFSNDIN-NKVFELIHD-SNEKLGGVLAVDTLID------ELPNQTSRLANYLRVLIDIEVMRAAATTLGKL--AVLTSEFVEFEVKTCIEWLTQEYRKHASLLIISSY--------------PFVNSILDNIWRAL------------------------RKLVIRTDAAVTLGKCLTSSLTKKWVQRLFNGCIHGTLLVYRQLVSLELK----DKYEEIYETTMKHRDNIIIRKEIYAIIPVLA--ADPKLFTQKYLDATMIHYLTLLLSDKGPILVSIGDIAYHI--GPYLDAIVENLRDGLETKFKTRKEK--DIFYCVSKLACAVRPLLAKYLNRGLLDA------SDHMQETLLVICEKIS--MEVTVNEKLLNIICLVLSGEKFRPPGSPTPMKSLSSETARAYRDQSLLRKTGTLDAMILRKALRMLSDIKPKYSLTEFIRRVIISYMEHDNMQVRKLAALTSCDLFVK-------SLYALNVVSEVLSKLLTVAITDPVAEIRLEILKHLDR-----LSQPDNTKLLFMALNDEVFAIQMEAMKICGRL------AVIPSLRKTLIQLLTELKHSKMTRKKEECASLLCTLISSSSDVTKPYLEPVIEILLPS------------------AVASTALKALGEISVVGGEDMLPFLDELMPLIID-TFQDQSNSFKRGAALKALGQLAASS----YPELLGVLINIL-----SQNIRRETVRLIGILGALDPYKHREVDVALLMQGEE---------------------YYPTVVIGVLMKILKDSIHHSTVIQAIMHIFQTM--------FLKQIIPGFILVMHPPSLLELYFQQLSVLISIVKQHIRPHVTEIVDVIS-------------LTIISVIESLSRALEGEFNPHLPTILSLFLDVLEN-------KKVVSVRILKSLVVFGPHLEEYVHLVLPTIIKLSSG-----------------------------NLKKAAIITIGRLSKNNA----------------LEMSSRIVQALVRVLNETEYVKATMNTLSLLLLQLNIDFTVFIPVINKTLTIYDRLVAKLLNNEP---LPTKIIIDKDFDLPNKEMDDVKV---ASKKLPVNQMVLKNAWDCSQQRTKEDWQEWIRRLSI---ESSSHALRACSG---------------------LAGIYYPLARELFNASFAQYQEDLIQSLCAALSST---------LLNLVEFMEHDDKPLPISISTLGEYAQRCHAYAKALHYKELEFIEEPSTSTIESLITINNQLHQTDAAIGILKHAQQHHDLQLKETWYEKLQRWDDALNAYNKREA-------------------------------------------------------EVTIGKMRSLHALGEWDQLSELAADKWASSE----------VKRAIAPLAAGAAWGLAQWDRVEQYIEVMKPQSPDKAFFDAILCLHRNNFEKAAEHIFSARDLLVTEMSAL----YNRAYGVVVRTQMVAELEEIIQYKKLPQG---SERRAMIRKTWNKRLLGCQ-NVDVWQRVLKVRSLVIKPKQDMKVWIKFANLCRKSGRLGLAQKTLNTLLEAPPPVVYAQLKYMWATGSQKEALNHLISFTSRMAHDLGLQHHIEDYTKLLARCFLKQGEWRVSLQPNWRLENPDAILGS---YLLATHFDKTWYKAWHNWALANFEVTSSLTQRIKDDKVPPTLNANDNFPPELVQRHVVPAIKGFFHSIALSQSS---LQDTLRLLTLWFKFGGIPEAAQAMHEGFGLIKIDNWLEVIPQLISRIHQPNQTVSRSLLSLLADLGKAHPQALVYPLTVAIKSDSVSRQRAALSIIDKMRMHSPKLVNQAELVSDELIRVAVLWHELWYEGLEDASRQFFGEHNTEKMFATLEPLHEMLKRGPETLREISFQNSFGRDLNDAYEWVLNYKRTKDINNLNQAWDIYYNVFRRISRKLPQLQTLDLQHVSPKLSAAKDLELAVPGTYH-AGKPIVRITHFEPVFTVISSKQRPRRVIIKGSDGKDYQYLLKSHEDIRQDNLVMQLFGLVNTLLQNDPESFQR-HLNIQQYPAIPLSPKTGLLGWVPNSDTFHVLIREHREASKIPLNIEHRIMLQM-----APDYDNLTLLQKVEVFTYALDNTKGQDLYKVLWLKSRSSESWLDRRTTYTRSLAVMSMVGYILGLGDRHPSNLMLDRITGKVVHIDFGDCFEAAILREKYPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMMVLRDNKESLMAILEAFAYDPLINWGFDLPTHAVMEQTGIDLPLANPSELLR-KGVISVEDAAKM----------------ELQQKAEVRNARATLVLKRIADKLTGNDFPRCQ--ELSVPDQVDKLIQQATSVENLCQHYIGWCS--

General information: TITO was launched using: RESULT: Template: 5FVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9777 -126517 -12.94 -60.91 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -12.94 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_5FVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FVM-query.scw PDB file : Tito_Scwrl_5FVM.pdb: